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Reads in SEQUEST summary html file and extracts relevant database search scores and peptide properties. Next, using search scores, computes a discriminant score (fval) for each search result. Writes output to .esi file.
This program has been adapted for Mascot results (converted to summary html format), and must be similarly adapted for parsing other database search programs.
Reads in .esi file containing search result discriminant score (fval) and relevant peptide properties, and iteratively learns the distributions of those values among correct and incorrect search results. Computes probabilities that each search result in the dataset is correct, and writes those results to .prob file
Reads in summary html file and .prob file, the writes computed probability as an extra first column to the designated output html file
Triggered by clicking on a probability link for a particular search result in the output to PeptideProphet analysis. Displays distributions of search result discriminant score (fval) and relevant peptide properties among correct and incorrect search results in the overall dataset. Overlaid on the discriminant score distribution is the value for the particular search result entry.